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  • Creatine Pyruvate,C7h13n3o5,Cas 55965-97-4

    1.METHOD OF ANALYSIS : Creatine Pyruvate Chemical Name : Creatine Pyruvate Structural formula : Molecular formula : C 7 H 13 N 3 O 5 Molecular weight : 219.20 CAS No. : 55965-97-4 Description: White crystalline powder. Loss on drying: Reference method: ChP Appendix VIII L Take about 1.0 g of the...
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    1.METHOD OF ANALYSIS Creatine Pyruvate

    Chemical  Name :  Creatine Pyruvate

    Structural formula

    3

    Molecular formula C7H13N3O5

    Molecular weight219.20

    CAS No. 55965-97-4

     

    Description:

    White crystalline powder.

     

    Loss on drying:

    Reference method: ChP Appendix VIII L

    Take about 1.0 g of the substance to be examined and place it into a dry and constant-weight flat weighing bottle. Dry it at 55℃ to a constant weight. Take out the bottle and cover well. Put the bottle into a dryer and cool to room temperature. Then weigh.

    The loss on drying should be not more than 0.5%.

     

    Residue on ignition:

    Reference method: ChP Appendix VIII N

    Accurately weigh 1.0 g of substance to be examined. Place it in a suitable crucible that previously has been ignited. Slowly ignite until the substance is thoroughly charred. Cool to room temperature. Moisten the sample with 0.5 mL of sulfuric acid. Heat at a low temperature until the steam of sulphuric acid disappears completely. And ignite at 600℃ until the carbon is consumed. Cool in a desiccator at room temperature for 1 hour, weigh, and calculate the percentage of residue.

    The residue on ignition should be not more than 0.1%.

     

    Iron:

    Reference method: ChP Appendix


    Standard ferric solution

    Weigh 0.863 g of ammonium ferric sulfate and place it into a 1000 ml volumetric flask. Dissolve with water .Add 2.5ml of sulfuric acid and dilute to the scale with appropriate water. Shake to make it homogeneous. Accurately transfer 10 ml of this solution and place it into a 100 ml volumetric flask. Dissolve and dilute to scale with water. Shake to make it homogeneous. (per 1 ml of this solution is equivalent to 10 µg of Fe).


    Test solution

    Take 1.0 g of the substance to be examined and dilute to 25 ml with water. Place it into a 50 ml Nessler’s color comparison tube .Add 10 ml of dilute hydrochloric acid and 50mg of ammonium persulfate .Dilute to 35 ml with water. Add 3ml of 30% ammonium thiocyanate solution and dilute to 50ml with water. Shake to make it homogeneous.


    Reference solution

    Transfer 1.0 ml of standard ferric solution and place it into a 50 ml Nessler’s color comparison tube. Add 10 ml of dilute hydrochloric acid and 50mg of ammonium persulfate.Dilute to 35 ml with water. Add 3ml of 30% ammonium thiocyanate solution and dilute to 50ml with water. Shake to make it homogeneous.


    Analysis

    Allow both the Test solution and Reference solution to stand for 5 min at a dark place. View downward against a black surface: the color of the Test solution should be not darker than that of the Reference solution.

    The Iron should be not more than 10 ppm.

     

    Heavy  metal :

    Reference method: ChP Appendix VIII H


    pH 3.5 Acetate buffer preparation

    Dissolve 25 g of ammonium acetate in 25 ml of water, and add 38 ml of 7 mol/L hydrochloric acid and exactly adjust the pH to 3.5 with 2 mol/L hydrochloric acid and 5 mol/L ammonium solution. Dilute to 100 ml with water.


    Standard lead preparation

    Weigh 0.160 g of lead nitrate and place it into a 1000 ml volumetric flask. Dissolve with 5 ml of nitric acid and 50 ml of water. Dilute to the scale with water. Shake to make it homogeneous. The stock solution is obtained.

    Accurately transfer 10 ml of this stock solution before use and place it into a 100 ml volumetric flask. Dilute to scale with water and shake to make it homogeneous. (per 1 ml of this solution is equivalent to 10 µg of Pb).


    Thioacetamide preparation

    Weigh 4 g of thioacetamide and dilute to 100 ml with water. Store it in a refrigerator. Take 5.0 ml of the mixture (including 15 ml 1 mol/L of sodium hydroxide, 5.0 ml of water and 20 ml of glycerin) and add 1ml of above thioacetamide solution. Heat it for 20 seconds on a water bath. Cool for immediate use.


    Analysis

    Take two 25 ml Nessler’s color comparison tubes.

    Tube 1: Add 1.0 ml of the Standard lead preparation and 2.0 ml of pH 3.5 Acetate buffer preparation into one tube. Dilute to 25 ml with water.

    Tube 2: Take 1.0 g of the substance to be examined and place it into another tube. Dissolve it with 15 ml of water. Add 2 ml of pH 3.5 Acetate buffer preparation and dilute to 25 ml with water.

    Respectively add 2 ml of thioacetamide solution in the Tube 1 and Tube 2. Shake to make it homogeneous and allow to stand for 2 min. View downward against a white surface.

    The color of Tube 2 should be not darker than Tube 1.

    The heavy metal should be not more than 10 ppm.

     

    Arsenic:

    Reference method: ChP Appendix VIII J


    Standard arsenic preparation

    Accurately weigh 0.132 g of arsenic trioxide and place it into a 1000 ml volumetric flask. Add 5 ml of 20% sodium hydroxide and neutralize with appropriate dilute sulfuric acid. Add 10 ml of dilute sulfuric acid and dilute to the scale with water. Shake to make it homogeneous. Accurately transfer 10 ml of above solution and place it into a 1000 ml volumetric flask. Add 10 ml of dilute sulfuric acid and dilute to scale with water. Shake to make it homogeneous. (per 1 ml of this solution is equivalent to 1µg of As).


    Instrument

    Join the equipment according to the requirement. Stuff a gas tube with 60 mg of lead acetate cotton (the height of lead acetate cotton in the tube should be 60 ~ 80 mm). Put a mercuric bromide test paper on the top of the plug and cover the plug tightly.


    Analysis

    Take 1.0 g of the substance to be examined and place it into a reaction bottle. Dissolve with 23 ml of water. Add 5 ml of hydrochloric acid, 5 ml of potassium iodide and 5 drops of acidic stannous chloride. Allow to stand for 10 min at room temperature and add 2 g of zinc. Insert the gas tube to the reaction bottle immediately. And put the bottle into a water-bath at 25 ~ 40℃ for 45 min. Take out the mercuric bromide test paper.

    The color of arsenic spot should be not darker than that of Standard arsenic preparation.

    The arsenic should be not more than 1 ppm.

     

    Pyruvic Ion  (on dried basis):

    Reference method: ChP Appendix V D

    Chromatographic conditions

    Column: Octadecyl silica gel

    Mobile phase:    Tetraethyl ammonium bromide (0.4%) : Acetonitrile =25 : 1 (V/V)

    Detector:  230 nm

    Mobile phase Flow rate:  1.0 ml/min


    Analysis

    Accurately weigh 15 ~ 20 mg of the substance to be examined and place it into a 50 ml volumetric flask. Dissolve and dilute to the scale with the Mobile phase. Shake to make it homogeneous. Accurately inject 20 µL into the chromatography and record the chromatogram.

    Determine the sodium pyruvate reference as above described procedure.


    Calculations

    Calculate the assay of pyruvic radical by external standard method:

    1

    In which,

    AS: area of the principal peak obtained with the sample chromatogram;

    MR: weight of the sodium pyruvate reference, mg;

    X: assay of pyruvic radical in sodium pyruvate reference;

    AR: area of the principal peak obtained with the sodium pyruvate reference chromatogram;

    MS: weight of the substance to be examined, mg.

    The assay of pyruvic radical should be not less than 30.0% (on dried basis).

     

    Creatine:

    Reference method: ChP Appendix V D

    Chromatographic conditions

    Column:  Octadecyl silica gel

    Mobile phase:    Tetraethyl ammonium bromide (0.4%) : Acetonitrile =25 : 1 (V/V)

    Detector:  220 nm

    Mobile phase Flow rate:  0.5 ml/min


    Analysis

    Accurately weigh about 30mg of the substance to be examined and place it into a 50 ml volumetric flask. Dissolve and dilute to the scale with the Mobile phase. Shake to make it homogeneous. Accurately inject 20 µl into the chromatography and record the chromatogram.

    Determine the creatine reference as above described procedure.


    Calculations

    Calculate the assay of creatine by external standard method:

    QQ截图20181108141709

    In which,

    AS: area of the principal peak obtained with the sample chromatogram;

    MR: weight of the creatine reference, mg;

    X: assay of creatine in creatine reference;

    Version

    Date

    Compiled by

    Revised by

    Approved by

    AR: area of the principal peak obtained with the creatine reference chromatogram;

    MS: weight of the substance to be examined, mg.

    The assay of creatine should be not less than 58.0% (on dried basis).



    2.Creatine Pyruvate The enterprise standard

    CAS#  55965-97-4

    Molecular formulaC7H13N3O5

    Molecular weight  219.20

    Structural formula

    4

     

    Description

      Creatine pyruvate is a novel form of creatine that is designed to enhance creatine’s absorption over the traditional form of creatine, namely, creatine monohydrate. Creatine pyruvate is 60% creatine and 40% pyruvate by weight.

     

    Function

    Creatine pyruvate has the advantage of being better soluble in water than creatine monohydrate and thus can be sold as a component of sports drinks or in the form of effervescent tablets. After ingestion, the creatine-pyruvate molecule in the acid environment of the stomach rapidly splits to form creatine and pyruvate, and thus from a theoretical point of view might also combine the beneficial effects of creatine supplementation on the one hand, and the presumed ergogenic action of pyruvate on the other hand.


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